p4xTRE1-Luc
(Plasmid #16535)

• Depositing Lab: Bert Vogelstein
• Publication: He et al Cell. 1999 Oct 29. 99(3):335-45.

Backbone

• Vector backbone: pBV-Luc
• Backbone manufacturer: Vogelstein Lab
• Backbone size w/o insert (bp): 4867
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 4xTRE1
• Alt name: Putative Tcf-4-binding sites
• Tag / Fusion Protein:
  • Luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: SmaI (not destroyed)
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: LucNrev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains 4 copies of putative Tcf-4-binding sites TRE1 (ATGTCTTTGTACTCG). The following oligonucleotide pairs were used for dimerization to construct corresponding reporters in pBV-Luc: 5'-CTAGCATGTC TTTGTACTCG ATGTCTTTGT ACTCG-3' and 5'-CTAGCGAGTA CAAAGACATC GAGTACAAAG ACATG-3' for p4XTRE1-Luc.