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Addgene

AAVS1-Puro-CAG-fl-STOP-fl-ZEB2-GFP-Flag-WPRE-poly(A) Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb extend ladder and a digestion of the plasmid AAVS1-Puro-CAG-fl-STOP-fl-ZEB2-GFP-Flag-WPREpoly(A) with the following enzymes: MluI-HF, NheI-HF, ScaI-HF, and KpnI-HF.

Expected bands:
MluI-HF/NheI-HF 10994 and 4199 bp
ScaI-HF/KpnI-HF 11685, 2398, and 1110 bp

We observed the expected bands for these digests.

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