StrKDEL-IRES-mannosidase II-mTagBFP2
(Plasmid #165460)

• Purpose: Expressed mannII-BFP simultaneously with a RUSH Hook Str-KDEL.
• Depositing Lab: David Stephens
• Depositing Organization: University of Bristol
  Located in United Kingdom of Great Britain and Northern Ireland
• Publication: McCaughey et al J Cell Sci. 2021 Aug 5. pii: 271201. doi: 10.1242/jcs.259075.
• Citations: 1 Article

Backbone

• Vector backbone: pIRESneo3
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Partial ManII-mTag2BFP fusion
• Alt name: This contains only amino acids 1 to 116 of ManII
• Species: M. musculus (mouse)
• Mutation: contains only amino acids 1 to 116 of ManII
• GenBank ID: NM_008549.2
• Entrez Gene: Man2a1 (a.k.a. Mana-2, Mana2, Map-2)
• Promoter: CMV
• Tags / Fusion Proteins:
  • mTag2BFP (C terminal on insert)
  • Str-KDEL (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: FseI (unknown if destroyed)
• 5′ sequencing primer: CMV

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Original clone was Plasmid #65253 from Franck Perez. mScarlet-i was synthesized from published sequence.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is a derivative of StrKDEL-IRES-ManII-mScarlet-i (Plasmid #117274)

DNA (Catalog # 165460-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 165460-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.