pCMV3Tag8li-hTXLNA-HA
(Plasmid #165472)

• Purpose: Human TXLNA mammalian expression vector with C-terminal HA tag.
• Depositing Lab: Martin Dorf
• Depositing Organization: Harvard Medical School
  Located in United States of America
• Publication: Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub 2011 Sep 8.

Backbone

• Vector backbone: pCMV-3Tag-8 modified MCS
• Backbone manufacturer: Stratagene /Agilent
• Backbone size w/o insert (bp): 5200
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: taxilin alpha
• Alt name: TXLNA, IL14, TXLN
• Alt name: BC080578
• Species: H. sapiens (human)
• Insert Size (bp): 1638
• Entrez Gene: TXLNA (a.k.a. IL14, TXLN)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: CMV, (T3)
• 3′ sequencing primer: T7, BGH polyA reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Open Biosystems clone: Human MGC Verified FL cDNA (IRAU) Clone Id: 6598234

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Backbone contains new multiple cloning site and expresses c-terminal HA epitope instead of FLAGs. Probably: Xhoi-Noti cloned: Xhoi-Kozak sequence-[TXLNA CDS 1638 bp, no stop]-Noti-a-Clai-HA epitope tag-ctt-STOP

DNA (Catalog # 165472-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 165472-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.