PurposeType II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||165507||Standard format: Plasmid sent in bacteria as agar stab||1||$85 *|
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Vector typeSynthetic Biology
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameNotI (GC-rich recognition site)
- Cloning method Restriction Enzyme
- 5′ cloning site AATG (unknown if destroyed)
- 3′ cloning site GCTT (unknown if destroyed)
- 5′ sequencing primer M13 forward
- 3′ sequencing primer M13 reverse (Common Sequencing Primers)
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
Type II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1.
The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information and protocols can be found at https://stanford.freegenes.org/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pOpen-NotIR (GC) was a gift from Drew Endy & Jennifer Molloy & FreeGenes Project (Addgene plasmid # 165507 ; http://n2t.net/addgene:165507 ; RRID:Addgene_165507)