pTBRII / CEP-Zeo / Hygro
(Plasmid #16622)

• Depositing Lab: Bert Vogelstein
• Depositing Organization: Johns Hopkins University and School of Medicine
  Located in the United States of America
• Publication: Zhou et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2412-6.
• Citations: 2 Articles

Backbone

• Vector backbone: pCEP4
• Backbone manufacturer: Invitrogen
• Modifications to backbone: pCMV-Zeo cassette inserted into pCEP4
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TGF beta type II receptor
• Alt name: TGFbRII
• Alt name: transforming growth factor, beta receptor II
• Species: H. sapiens (human)
• Entrez Gene: TGFBR2 (a.k.a. AAT3, FAA3, LDS1B, LDS2, LDS2B, MFS2, RIIC, TAAD2, TBR-ii, TBRII, TGFR-2, TGFbeta-RII, tbetaR-II)
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: pGEX3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: TGFbRII cDNA generously provided by Dr. Harvey Lodish and Dr. Robert Weinberg

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

An expression construct for the TGF-beta RII gene was constructed by placing a TGF-beta RII cDNA between a cytomegalovirus (CMV) promoter and an simian virus 40 polyadenylation signal.

Please note that according to the map, this plasmid has 2 CMV promoters, so sequence confirmation with this primer may have limited success. Restriction digest with BamHI/HindIII is recommended for plasmid confirmation.

DNA (Catalog # 16622-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 16622-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.