Purposeuse as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmid
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||166246||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 3212
Vector typeBacterial Expression
Growth in Bacteria
Growth Strain(s)NEB Stable
Growth instructionsThis plasmid can be cured by growth at 37C because it has temperature-sensitive replicon.
Insert Size (bp)4618
- Promoter Ec-TTL-P112 and BBa_J23116
- Cloning method Gibson Cloning
- 5′ sequencing primer catcgatttatgccacctgacgtc
- 3′ sequencing primer tataaacgcagaaaggcccacc (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAUX_OL was a gift from Domitilla Del Vecchio (Addgene plasmid # 166246 ; http://n2t.net/addgene:166246 ; RRID:Addgene_166246)
For your References section:dCas9 regulator to neutralize competition in CRISPRi circuits. Huang HH, Bellato M, Qian Y, Cardenas P, Pasotti L, Magni P, Del Vecchio D. Nat Commun. 2021 Mar 16;12(1):1692. doi: 10.1038/s41467-021-21772-6. 10.1038/s41467-021-21772-6 PubMed 33727557