pBS L30 mRuby3-Rab10
(Plasmid
#166429)
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PurposeRab10 fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.
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Depositing Lab
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Depositing OrganizationCNRS, Delegation Occitanie Est
Located in France -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 166429 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 166429-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 166429-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepBS
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Modifications to backboneModified for mammalian expression using the promoter of the mouse ribosomal protein L30 gene ("weak promoter") and a HSV TK polyA tail
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRab10
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SpeciesH. sapiens (human)
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GenBank ID10890 NC_000002.12
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Entrez GeneRAB10
- Promoter L30 weak promoter
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Tag
/ Fusion Protein
- mRuby3 (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13pUC-fwd
- 3′ sequencing primer T3
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 166429-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 166429-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS L30 mRuby3-Rab10 was a gift from Raphael Gaudin (Addgene plasmid # 166429 ; http://n2t.net/addgene:166429 ; RRID:Addgene_166429)
