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(Plasmid #166577)


Item Catalog # Description Quantity Price (USD)
Plasmid 166577 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pTrcHis C
  • Backbone manufacturer
    Thermo Fisher Scientific, Carlsbad, CA
  • Total vector size (bp) 5630
  • Modifications to backbone
    includes an N-terminal hexahistidine tag (MGGSHHHHHHGMASMTGGQQMGRDLYDDDD- KDRWIRPRA) before the insert.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Use BL21-Star (DE3), or other suitable strain for expression. 37C for 5h growing after induction. At lower temperature can be O/N. IPTG as the inducing agent
  • Copy number
    High Copy


  • Gene/Insert name
    Tln1 Head (1-400)
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    residues 1-400 only
  • Entrez Gene
    TLN1 (a.k.a. ILWEQ, TLN, talin-1)
  • Tag / Fusion Protein
    • His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    t1-400 was a gift from Vesa Hytönen (Addgene plasmid # 166577 ; ; RRID:Addgene_166577)
  • For your References section:

    Crystal structure of the FERM-folded talin head reveals the determinants for integrin binding. Zhang P, Azizi L, Kukkurainen S, Gao T, Baikoghli M, Jacquier MC, Sun Y, Maatta JAE, Cheng RH, Wehrle-Haller B, Hytonen VP, Wu J. Proc Natl Acad Sci U S A. 2020 Dec 22;117(51):32402-32412. doi: 10.1073/pnas.2014583117. Epub 2020 Dec 7. 10.1073/pnas.2014583117 PubMed 33288722