pGY466
(Plasmid #166661)

• Purpose: For light-inducible Cre recombinase (LiCre) expression under the Met17 promoter in yeast
• Depositing Lab: Gaël Yvert
• Publication: Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi: 10.7554/eLife.61268.

Backbone

• Vector backbone: pSH63
• Backbone manufacturer: Euroscarf
• Vector type: Yeast Expression, Cre/Lox
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LiCre
• Alt name: AsLOV2-CreE340AD341A
• Species: Synthetic; Avena sativa, Bacteriopage P2
• Mutation: Cre: E340A, D341A
• Promoter: MET17

Cloning Information

• Cloning method: Ligation Independent Cloning

Resource Information

• Supplemental Documents:
  • pGY466.gb
• A portion of this plasmid was derived from a plasmid made by: Vector pSH63 originates from Gueldener et al. Nucleic Acids Research, 2002, Vol. 30, No. 6 e23

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains 6 nucleotide discrepancies in the Met17 promoter sequence. These discrepancies are not known to affect plasmid function.