Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16688||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Vector typeXenopus Expression
Growth in Bacteria
SpeciesX. laevis (frog)
Entrez Geneszl (a.k.a. XELAEV_18021029mg)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer EBV reverse (Common Sequencing Primers)
This encodes a secreted
antagonist of Xwnt-8.
The entire open reading frame of sizzled was ampliﬁed by low-cycle number PCR using Pfu polymerase and cloned as a
BamHI-XhoI fragment into pCS2+ .
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2+ Sizzled was a gift from Marc Kirschner (Addgene plasmid # 16688 ; http://n2t.net/addgene:16688 ; RRID:Addgene_16688)
For your References section:Sizzled: a secreted Xwnt8 antagonist expressed in the ventral marginal zone of Xenopus embryos. Salic AN, Kroll KL, Evans LM, Kirschner MW. Development. 1997 Dec . 124(23):4739-48. PubMed 9428410