pCDNA3_ReBphP-PCM_IRES_sfGFP
(Plasmid #166917)

• Purpose: Express ReBphP-PCM and sfGFP
• Depositing Lab: Andre Stiel
• Publication: Mishra et al Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi: 10.1126/sciadv.aaz6293. eCollection 2020 Jun.

Backbone

• Vector backbone: pcDNA3.0
• Backbone manufacturer: Thermo Fisher Scientific
• Backbone size w/o insert (bp): 5446
• Total vector size (bp): 8340
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: ReBphP-PCM
• Species: Rhizobium etli
• Insert Size (bp): 1575
• GenBank ID:
• Promoter: CMV

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: Xho1 (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: gcgtctaagttacgggaag

Gene/Insert 2

• Gene/Insert name: sfGFP
• Alt name: superfolder GFP
• Species: Aequorea victoria
• Insert Size (bp): 720
• GenBank ID:
• Promoter: CMV

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (unknown if destroyed)
• 3′ cloning site: Notl (unknown if destroyed)
• 5′ sequencing primer: gactactttgggtgtccg
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • ReBphP-PCM_IRES_GFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

ReBphP-PCM is codon-optimized for mammalian expression