GFP-p58
(Plasmid
#166942)
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Purposemammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with GFP
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Depositing Lab
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Depositing OrganizationHHMI, Janelia Research Campus
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 166942 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 166942-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 166942-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneGFP
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep58
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Alt nameERGIC-53
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Alt nameLMAN1
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SpeciesR. norvegicus (rat)
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Entrez GeneLman1 (a.k.a. p58)
- Promoter CMV
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Tags
/ Fusion Proteins
- GFP (N terminal on insert)
- chicken lysozyme signal peptide (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV F
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note there are two mutations in p58-L405Q, R419S Depositor confirms these do not affect plasmid function.
DNA (Catalog # 166942-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 166942-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-p58 was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 166942 ; http://n2t.net/addgene:166942 ; RRID:Addgene_166942) -
For your References section:
Maintenance of Golgi structure and function depends on the integrity of ER export. Ward TH, Polishchuk RS, Caplan S, Hirschberg K, Lippincott-Schwartz J. J Cell Biol. 2001 Nov 12;155(4):557-70. doi: 10.1083/jcb.200107045. Epub 2001 Nov 12. 10.1083/jcb.200107045 PubMed 11706049
