Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #166976)


Item Catalog # Description Quantity Price (USD)
Plasmid 166976 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Chris Lima
  • Backbone size w/o insert (bp) 5633
  • Total vector size (bp) 6869
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    C. elegans (nematode)
  • Mutation
    FBF-2 PUM RNA binding domain; amino acids 164-575; changed cysteine 363 to serine, changed glutamine 367 to serine
  • Entrez Gene
    fbf-2 (a.k.a. CELE_F21H12.5)
  • Promoter T7
  • Tag / Fusion Protein
    • His-SUMO (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSMT3-FBF2 SS/R was a gift from Traci Hall (Addgene plasmid # 166976 ; ; RRID:Addgene_166976)
  • For your References section:

    Engineering a conserved RNA regulatory protein repurposes its biological function in vivo. Bhat VD, McCann KL, Wang Y, Fonseca DR, Shukla T, Alexander JC, Qiu C, Wickens M, Lo TW, Tanaka Hall TM, Campbell ZT. Elife. 2019 Jan 17;8. pii: 43788. doi: 10.7554/eLife.43788. 10.7554/eLife.43788 PubMed 30652968