pgRNA_CV2
(Plasmid
#167165)
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Purpose(Empty Backbone) pgRNA_CV2 is derived from gRNA_cloning vector (Addgene plasmid ID: 41824) by adding about 80 bps from the sgRNA sequence as well as an AgeI site. In the literature, sgRNA_AL is used as an alias.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 167165 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonegRNA_Cloning Vector (Plasmid #41824)
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Backbone manufacturerDepositing Lab: George Church
- Backbone size (bp) 3914
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Modifications to backboneAgeI site was added to simplify cloning by Gibson assembly
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Vector typeMammalian Expression, CRISPR
- Promoter U6 promoter
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pgRNA_CV2 was a gift from Alexander Loewer (Addgene plasmid # 167165 ; http://n2t.net/addgene:167165 ; RRID:Addgene_167165) -
For your References section:
PCNA-Mediated Degradation of p21 Coordinates the DNA Damage Response and Cell Cycle Regulation in Individual Cells. Sheng C, Mendler IH, Rieke S, Snyder P, Jentsch M, Friedrich D, Drossel B, Loewer A. Cell Rep. 2019 Apr 2;27(1):48-58.e7. doi: 10.1016/j.celrep.2019.03.031. 10.1016/j.celrep.2019.03.031 PubMed 30943414
Map uploaded by the depositor.
