pDONR221_SMAD2_HAe2_Bsd
(Plasmid
#167166)
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PurposeBlasticidin selection cassette flanked by homology arms from human SMAD2 exon 2 for use in creating CRISPR-Cas9 mediated gene knockout.
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Depositing Lab
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Depositing OrganizationTechnische Universitaet Darmstadt
Located in Germany -
Publication
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Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 167166 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 167166-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 167166-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepDONR221
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2471
- Total vector size (bp) 4762
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Vector typeRepair template for gene editing in human cells
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRepair template to knockout SMAD2 by inserting a blasticidin resistance cassette
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SpeciesH. sapiens (human)
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Insert Size (bp)2325
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GenBank IDNG_029946.1
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Entrez GeneSMAD2 (a.k.a. CHTD8, JV18, JV18-1, LDS6, MADH2, MADR2, hMAD-2, hSMAD2)
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Tag
/ Fusion Protein
- blasticidin
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13 forward
- 3′ sequencing primer M13 reverse
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 167166-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 167166-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR221_SMAD2_HAe2_Bsd was a gift from Alexander Loewer (Addgene plasmid # 167166 ; http://n2t.net/addgene:167166 ; RRID:Addgene_167166)
Map uploaded by the depositor.
