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(Plasmid #167256)


Item Catalog # Description Quantity Price (USD)
Plasmid 167256 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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  • Vector backbone
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Nsp1 (residues 13-127)
  • Species
  • Mutation
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Tag / Fusion Protein
    • His6-TEV site (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMCSG53_Nsp1 was a gift from Alexei Savchenko (Addgene plasmid # 167256 ; ; RRID:Addgene_167256)
  • For your References section:

    Structural characterization of nonstructural protein 1 from SARS-CoV-2. Semper C, Watanabe N, Savchenko A. iScience. 2021 Jan 22;24(1):101903. doi: 10.1016/j.isci.2020.101903. Epub 2020 Dec 7. 10.1016/j.isci.2020.101903 PubMed 33319167