pcDNA3.1/ZMYM2-FGFR1
(Plasmid #167486)

• Purpose: Mammalian expression vector for the ZMYM2-FGFR1 fusion gene associated with myeloid and lymphoid neoplasms
• Depositing Lab: Nicholas Cross
• Publication: Smedley et al Neoplasia. 1999 Oct;1(4):349-55. doi: 10.1038/sj.neo.7900035.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5427
• Total vector size (bp): 9497
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ZMYM2-FGFR1
• Alt name: ZNF198-FGFR1
• Species: H. sapiens (human)
• Insert Size (bp): 4075
• GenBank ID: NM_003453.6 NM_023110.3
• Entrez Gene: FGFR1 (a.k.a. BFGFR, CD331, CEK, ECCL, FGFBR, FGFR-1, FLG, FLT-2, FLT2, HBGFR, HH2, HRTFDS, KAL2, N-SAM, OGD, bFGF-R-1)
• Entrez Gene: ZMYM2 (a.k.a. FIM, MYM, NECRC, RAMP, SCLL, ZNF198)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHl (not destroyed)
• 3′ cloning site: BamHl (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry