pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G Citations (5)
Originally described in: A functional genetic toolbox for human tissue-derived organoids.Sun D, Evans L, Perrone F, Sokleva V, Lim K, Rezakhani S, Lutolf M, Zilbauer M, Rawlins EL eLife. 2021 Oct 6;10. pii: 67886. doi: 10.7554/eLife.67886. PubMed Journal
Articles Citing pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G
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| SOX9 maintains human foetal lung tip progenitor state by enhancing WNT and RTK signalling. Sun D, Llora Batlle O, van den Ameele J, Thomas JC, He P, Lim K, Tang W, Xu C, Meyer KB, Teichmann SA, Marioni JC, Jackson SP, Brand AH, Rawlins EL. EMBO J. 2022 Nov 2;41(21):e111338. doi: 10.15252/embj.2022111338. Epub 2022 Sep 19. PubMed |
Organoid modeling of human fetal lung alveolar development reveals mechanisms of cell fate patterning and neonatal respiratory disease.
Lim K, Donovan APA, Tang W, Sun D, He P, Pett JP, Teichmann SA, Marioni JC, Meyer KB, Brand AH, Rawlins EL.
Cell Stem Cell. 2023 Jan 5;30(1):20-37.e9. doi: 10.1016/j.stem.2022.11.013. Epub 2022 Dec 8.
PubMed
Associated Plasmids |
| A novel human fetal lung-derived alveolar organoid model reveals mechanisms of surfactant protein C maturation relevant to interstitial lung disease. Lim K, Rutherford EN, Delpiano L, He P, Lin W, Sun D, Van den Boomen DJH, Edgar JR, Bang JH, Predeus A, Teichmann SA, Marioni JC, Matesic LE, Lee JH, Lehner PJ, Marciniak SJ, Rawlins EL, Dickens JA. EMBO J. 2025 Feb;44(3):639-664. doi: 10.1038/s44318-024-00328-6. Epub 2025 Jan 15. PubMed |
| Single-cell multiomics reveals a gene regulatory circuit driving leukemia cell differentiation. Tian X, Zhang L, Xiang G, Tang Y, Zhu P, Yu S, Jiang F, Wang S, Wang J, Dai Y, Zheng D, Wang J, Weng X, Wang S, Tan Y, Liu F. Oncogene. 2025 May;44(19):1350-1360. doi: 10.1038/s41388-025-03309-z. Epub 2025 Feb 22. PubMed |
| Hypoxia promotes airway differentiation in the human lung epithelium. Dong Z, Wit N, Agarwal A, Reid AJ, Dubal D, Beier S, Mahbubani KT, Saeb-Parsy K, van den Ameele J, Nathan JA, Rawlins EL. Cell Stem Cell. 2025 Nov 6;32(11):1705-1722.e9. doi: 10.1016/j.stem.2025.09.007. Epub 2025 Oct 10. PubMed |
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