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(Plasmid #167935)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167935 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number


  • Gene/Insert name
  • Species
  • Insert Size (bp)
  • Promoter tetON

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer gacagatccattcgattagtg
  • 3′ sequencing primer GCTCAAGGGGCTTCATGATG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

KRAB-dCas9 cloned from pAAVS1-NDi-CRISPRi (Gen1) (Addgene Plasmid #73497).

Please visit for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G was a gift from Emma Rawlins (Addgene plasmid # 167935 ; ; RRID:Addgene_167935)
  • For your References section:

    A functional genetic toolbox for human tissue-derived organoids. Sun D, Evans L, Perrone F, Sokleva V, Lim K, Rezakhani S, Lutolf M, Zilbauer M, Rawlins EL. Elife. 2021 Oct 6;10. pii: 67886. doi: 10.7554/eLife.67886. 10.7554/eLife.67886 PubMed 34612202