A functional genetic toolbox for human tissue-derived organoids.
Sun D, Evans L, Perrone F, Sokleva V, Lim K, Rezakhani S, Lutolf M, Zilbauer M, Rawlins EL
Elife. 2021 Oct 6;10. pii: 67886. doi: 10.7554/eLife.67886.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 167935 | pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G | Inducible CRISPRi expression |
| 167936 | pLenti-U6-gRNA-EF1a-EGFP-CAAX | gRNA entry vector |
| 167937 | pLenti-tetON-dxCas9(3.7)-VPR-EF1a-TagRFP-2A-tet3G | Inducible CRISPRa expression |
| 167942 | pLenti-tetON-NKX2-1-EF1a-TagRFP-2A-tet3G | Inducible NKX2-1 overexpression |
| 167943 | SFTPC-T2A-Rox-EF1a-Rox-Venus-NLS repair template | SFTPC reporter targeting repair template |
| 167968 | CMV-Dre-T2A-TagRFP | Transient Dre expression |
| 167969 | SOX2 KO (T2A-H2B-EGFP) | SOX2 CDS replacement with T2A-H2B-EGFP |
| 167971 | TP63-T2A-Rox-EF1a-Rox-Venus-NLS repair template | Tp63 reporter targeting repair template |
| 167972 | SOX9-T2A-H2B-EGFP repair template | SOX9 reporter repair template |
| 167973 | AAVS1-EF1a-mTagRFP-T-CAAX | AAVS1 targeting repair template |