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A functional genetic toolbox for human tissue-derived organoids.

Sun D, Evans L, Perrone F, Sokleva V, Lim K, Rezakhani S, Lutolf M, Zilbauer M, Rawlins EL
Elife. 2021 Oct 6;10. pii: 67886. doi: 10.7554/eLife.67886. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
167935pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3GInducible CRISPRi expression
167936pLenti-U6-gRNA-EF1a-EGFP-CAAXgRNA entry vector
167937pLenti-tetON-dxCas9(3.7)-VPR-EF1a-TagRFP-2A-tet3GInducible CRISPRa expression
167942pLenti-tetON-NKX2-1-EF1a-TagRFP-2A-tet3GInducible NKX2-1 overexpression
167943SFTPC-T2A-Rox-EF1a-Rox-Venus-NLS repair templateSFTPC reporter targeting repair template
167968CMV-Dre-T2A-TagRFPTransient Dre expression
167969SOX2 KO (T2A-H2B-EGFP)SOX2 CDS replacement with T2A-H2B-EGFP
167971TP63-T2A-Rox-EF1a-Rox-Venus-NLS repair templateTp63 reporter targeting repair template
167972SOX9-T2A-H2B-EGFP repair templateSOX9 reporter repair template
167973AAVS1-EF1a-mTagRFP-T-CAAXAAVS1 targeting repair template

Antibodies from Article