XE224 human flag-Chibby-pCS3+FLT
(Plasmid #16824)

• Depositing Lab: Randall Moon
• Publication: Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

Backbone

• Vector backbone: pCS3+FLT
• Backbone manufacturer: ATCC
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: flag-Chibby
• Alt name: cby
• Species: H. sapiens (human)
• Insert Size (bp): 1000
• GenBank ID: AL050345
• Entrez Gene: CBY1 (a.k.a. C22orf2, CBY, Chibby1, HS508I15A, PGEA1, PIGEA-14, PIGEA14, arb1)
• Tag / Fusion Protein:
  • flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (unknown if destroyed)
• 3′ cloning site: Xho1 (unknown if destroyed)
• 5′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The full coding sequence as well as 3' UTR of Human Chibby, ~1kb, was amplified by PCR and subcloned into the EcoR1 and Xho1 sites of pCS3+FLT, providing a flag tag.

Reference: K. Takemaru et al., Nature 422: 905 (2003)