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pMRX-IPU-FLAG-NEK9T210A
(Plasmid #168270)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168270 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 168270-D50 50 μg of DNA in Tris buffer $495
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DNA 168270-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pMRX-IP
  • Backbone manufacturer
    Dr.Shoji Yamaoka of Tokyo Medical and Dental University
  • Backbone size w/o insert (bp) 6100
  • Total vector size (bp) 9177
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NEK9
  • Alt name
    NIMA related kinase 9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2973
  • Mutation
    changed threonine 210 to alanine
  • GenBank ID
    NM_001329237
  • Entrez Gene
    NEK9 (a.k.a. APUG, LCCS10, NC, NERCC, NERCC1)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ttcaggctcgGGATCCtcggtgctgggcgag
  • 3′ sequencing primer acccagcctctagGAATTCgggtCTCGAGaGCGGC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    It has the pMX backbone

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMRX-IPU-FLAG-NEK9T210A was a gift from Noboru Mizushima (Addgene plasmid # 168270 ; http://n2t.net/addgene:168270 ; RRID:Addgene_168270)

  • For your References section:

    NEK9 regulates primary cilia formation by acting as a selective autophagy adaptor for MYH9/myosin IIA. Yamamoto Y, Chino H, Tsukamoto S, Ode KL, Ueda HR, Mizushima N. Nat Commun. 2021 Jun 2;12(1):3292. doi: 10.1038/s41467-021-23599-7. 10.1038/s41467-021-23599-7 PubMed 34078910
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