pMRX-IPU-FLAG-NEK9T210A
(Plasmid #168270)

• Purpose: Expresses kinase-dead NEK9 mutant in mammalian cells
• Depositing Lab: Noboru Mizushima
• Publication: Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi: 10.1038/s41467-021-23599-7.

Backbone

• Vector backbone: pMRX-IP
• Backbone manufacturer: Dr.Shoji Yamaoka of Tokyo Medical and Dental University
• Backbone size w/o insert (bp): 6100
• Total vector size (bp): 9177
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NEK9
• Alt name: NIMA related kinase 9
• Species: H. sapiens (human)
• Insert Size (bp): 2973
• Mutation: changed threonine 210 to alanine
• GenBank ID: NM_001329237
• Entrez Gene: NEK9 (a.k.a. APUG, LCCS10, NC, NERCC, NERCC1)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ttcaggctcgGGATCCtcggtgctgggcgag
• 3′ sequencing primer: acccagcctctagGAATTCgggtCTCGAGaGCGGC

Resource Information

• Supplemental Documents:
  • pMRX-IPU-FLAG-NEK9T210A.gb
• A portion of this plasmid was derived from a plasmid made by: It has the pMX backbone

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry