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XE49 pt Beta-catenin-myc
(Plasmid #16840)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16840 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 16840-D50 50 μg of DNA in Tris buffer $495
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DNA 16840-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcS2+MT
  • Backbone size w/o insert (bp) 4352
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pt beta-catenin-myc
  • Alt name
    beta catenin
  • Species
    X. laevis (frog)
  • Mutation
    four point mutations introduced into the GSK-3 phosphorylation site
  • Entrez Gene
    ctnnb1.L (a.k.a. XELAEV_18031149mg, B-catenin, beta-catenin, ctnnb, ctnnb1, ctnnb1-a, ctnnb1-b)
  • Tag / Fusion Protein
    • 6x myc (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This Xenopus Beta-catenin has four point mutations introduced into the GSK-3 phosphorylation site as shown.
The construct is derived from construct XE-28 and is linearized, transcribed, and used as described there, and in the original citation.

Ref: Yost et al., Genes & Development, 10:1443-1454, 1996.

four mutations are amino acids S33A, S37A, T41A, S45A

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE49 pt Beta-catenin-myc was a gift from Randall Moon (Addgene plasmid # 16840 ; http://n2t.net/addgene:16840 ; RRID:Addgene_16840)

  • For your References section:

    The axis-inducing activity, stability, and subcellular distribution of beta-catenin is regulated in Xenopus embryos by glycogen synthase kinase 3. Yost C, Torres M, Miller JR, Huang E, Kimelman D, Moon RT. Genes Dev. 1996 Jun 15. 10(12):1443-54. 10.1101/gad.10.12.1443 PubMed 8666229
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