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Purposemaking transgenics expressing a UAS driven eGFP and NTR 2.0
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 158652 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbone(na) pTol2mini?
- Total vector size (bp) 8321
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Vector typeTol2-based transgenic expression in zebrafish/other
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name5xUAS:GAP-eGFP-P2A-NfsB_Vv F70A/F108Y;he:BFP
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SpeciesEscherichia coli
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MutationF70A/F108Y
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
5xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2 was a gift from Jeff Mumm (Addgene plasmid # 158652 ; http://n2t.net/addgene:158652 ; RRID:Addgene_158652) -
For your References section:
NTR 2.0: a rationally-engineered prodrug converting enzyme with substantially enhanced efficacy for targeted cell ablation. Sharrock AV, Mulligan TS, Hall KR, Williams EM, White DT, Zhang L, Matthews F, Nimmagadda S, Washington S, Le K , Meir-Levi D, Cox OL, Saxena MT, Calof AL, Lopez-Burks ME, Lander AD, Ding D, Ji H, Ackerley DF, Mumm JS. bioRxiv 10.1101/2020.05.22.111427
