p-preSufI(IAC)
(Plasmid #168516)

• Purpose: Modified form of pre-SufI in pET25b. With C-terminal HHHHHHC (6xHisC) extension and mutations C17I and C295A.
• Depositing Lab: Siegfried Musser
• Publication: Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi: 10.1371/journal.pone.0256715. eCollection 2021.

Backbone

• Vector backbone: pET25b
• Backbone manufacturer: Novagen (EMD Millipore)
• Backbone size w/o insert (bp): 5547
• Total vector size (bp): 6834
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pre-SufI(IAC)
• Species: Escherichia coli
• Insert Size (bp): 1412
• Mutation: C17I and C295A
• GenBank ID: CAD5996880.1
• Promoter: T7
• Tags / Fusion Proteins:
  • HSV tag (C terminal on insert)
  • 6xHisC tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 Fwd
• 3′ sequencing primer: T7 Terminator

Resource Information

• Supplemental Documents:
  • p-preSufI(IAC).gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid was originally reported as pSufI-IAC in Bageshwar et al. 2009 Mol. Microbiol., 74:209-226.

This plasmid has 6 silent mutations: L39L (CTG to CTA); P131P (CCT to CCC); L273L (TTG to CTG); G288G (GGT to GGC); A295A (GCA to GCG); and S313S (AGC to AGT).