XE3 Xwnt-5A Full Length
(Plasmid #16875)

• Depositing Lab: Randall Moon
• Publication: Xenopus Expression Plasmids (unpublished)

Backbone

• Vector backbone: pSP64T
• Backbone size w/o insert (bp): 3000
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Xwnt-5A
• Alt name: wnt5
• Alt name: xwnt
• Species: X. laevis (frog)
• Insert Size (bp): 3000
• Entrez Gene: wnt5a.L (a.k.a. Xwnt-5A, wnt-5a, wnt5a-a, wnt5a-b)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bgl II linker, inserted into Bgl II site (unknown if destroyed)
• 5′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert: cDNA from Melton lambda gt10 oocyte library.
Insert size: 3 Kb.
Transcription: linearize with Xba1 and transcribe with SP6.
Sense: plasmid prep #2 on 9/26/90.
Antisense: plasmid prep #7 on 9/26/90.

Plasmid History:
Constructed by Randall Moon on date of 9/15/90.
Notes for Use:
EcoR1 insert of lambda G6 of 2/11/90. Contains significant 5' UTR and is less translatable in vitro per ng RNA than Xwnt-5A Hind III/SP64T. However, the type of activity is the same in both constructs.

Citation: Moon et al., Development 119, 97 (1993)