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pAAV-GFAP-eLACCO1.1
(Plasmid #168790)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 168790 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eLacco1.1
  • Insert Size (bp)
    1821
  • Promoter GFAP
  • Tags / Fusion Proteins
    • GPI (C terminal on insert)
    • ER export (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer GFAP
  • 3′ sequencing primer WPRE
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Robert Campbell

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-GFAP-eLACCO1.1 was a gift from Marie-Eve Paquet (Addgene plasmid # 168790 ; http://n2t.net/addgene:168790 ; RRID:Addgene_168790)
  • For your References section:

    A genetically encoded fluorescent biosensor for extracellular L-lactate. Nasu Y, Murphy-Royal C, Wen Y, Haidey JN, Molina RS, Aggarwal A, Zhang S, Kamijo Y, Paquet ME, Podgorski K, Drobizhev M, Bains JS, Lemieux MJ, Gordon GR, Campbell RE. Nat Commun. 2021 Dec 6;12(1):7058. doi: 10.1038/s41467-021-27332-2. 10.1038/s41467-021-27332-2 PubMed 34873165