ZP62 Cystathionine beta-synthase
(Plasmid
#16880)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16880 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSPORT1
- Backbone size w/o insert (bp) 4100
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecystathionine beta-synthase
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SpeciesD. rerio (zebrafish)
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Entrez Genecbsb (a.k.a. cbs, cb442, wu:fb37g05, wu:fm61c07, wu:fq06c06, zgc:113704)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Zebrafish cystathionine beta-synthase cDNA (likely full-lenghth, at least contains full coding sequence) in pSPORT1. clone 6h9 from the microarray screen 1st round (Matthew clark normalized late somitogenesis / 24 hpf / liver library).
Expressed pretty ubiquitously early, but stronger in axail mesoderm during gastrulation. Later most prominently in eyes and somites.
For antisense: Asp718, SP6.
Citation: G. Weidinger et al Development 2005.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ZP62 Cystathionine beta-synthase was a gift from Randall Moon (Addgene plasmid # 16880 ; http://n2t.net/addgene:16880 ; RRID:Addgene_16880)