|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16883||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesD. rerio (zebrafish)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Clone 23f17 from the microarray screen 1st round (Matthew clark normalized late somitogenesis / 24 hpf / liver library). Noncoding, repetitive transcript. Similar and overlapping with many other regions in the genome. For antisense probe synthesis.
Expressed in paraxial and ventral mesoderm during gastrulation. Later in specific brain regions.
For antisense: EcoRI, SP6.
Citation: G Weidinger and R. T. Moon (2005). Development.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ZP59 23f17 was a gift from Randall Moon (Addgene plasmid # 16883 ; http://n2t.net/addgene:16883 ; RRID:Addgene_16883)