|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16895||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesD. rerio (zebrafish)
Entrez Genenlk1 (a.k.a. nlk, nlkb, zgc:111875)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
For antisense probe cut with SacII and transcribe with SP6. For sense probe cut with Sal1 and transcribe with T7.
Citation: C. J. Thorpe and R. T. Moon (2004). Development 131:2899-2909.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ZP47 Nlk1 was a gift from Randall Moon (Addgene plasmid # 16895 ; http://n2t.net/addgene:16895 ; RRID:Addgene_16895)
Map uploaded by the depositor.