pTorD-H6
(Plasmid #169039)

• Purpose: Escherichia coli TorD with C-terminal 6xHis in pET28a.
• Depositing Lab: Siegfried Musser
• Publication: Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi: 10.1371/journal.pone.0256715. eCollection 2021.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: EMD Biosciences
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 5870
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TorD-H6
• Species: Escherichia coli
• Insert Size (bp): 624
• Mutation: The mutation N at position 46 was changed back to the wildtype S.
• GenBank ID: CAA52096.1
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7 Fwd
• 3′ sequencing primer: T7 Terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The TorD-6xHis coding sequence was recovered from pQE80TorADhis, a plasmid obtained from Tracy Palmer and first reported in Dow et al., 2013 Biochem. J., 452:57-66.