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pH6-TEV-spTorA-GFP(C)
(Plasmid #169041)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 169041 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBAD24
  • Backbone manufacturer
    Beckwith Lab
  • Backbone size w/o insert (bp) 4542
  • Total vector size (bp) 5413
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H6-spTorA-GFP
  • Species
    Escherichia coli and Aequorea victoria
  • Insert Size (bp)
    900
  • Mutation
    The mut3 GFP variant with 5 additional mutations (Q80R, S147H, M153T, V163A, S202D). There are also 3 silent mutations at G65 (GGT instead of GGG), Q69 (CAA instead of CAG) and P75 (CCA instead of CCG). Internal NcoI site in GFP was deleted (CCATGG to CCCTGG).
  • GenBank ID
    CAD6011594.1 AAB18957.1
  • Promoter araBAD
  • Tags / Fusion Proteins
    • 6xHis (N terminal on insert)
    • TEV cleavage site (N terminal on insert)
    • spTorA (signal peptide of E. coli TorA) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer pBAD forward primer
  • 3′ sequencing primer pBAD reverse primer
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The spTorA-GFP coding sequence was recovered from p-spTorA-GFP-H6C, a plasmid originally reported as pTorA-GFP in Bageshwar & Musser 2007 J. Cell Biol., 179:87-99.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pH6-TEV-spTorA-GFP(C) was a gift from Siegfried Musser (Addgene plasmid # 169041 ; http://n2t.net/addgene:169041 ; RRID:Addgene_169041)
  • For your References section:

    Influence of the TorD signal peptide chaperone on Tat-dependent protein translocation. Bageshwar UK, DattaGupta A, Musser SM. PLoS One. 2021 Sep 9;16(9):e0256715. doi: 10.1371/journal.pone.0256715. eCollection 2021. 10.1371/journal.pone.0256715 PubMed 34499687