PurposeTESEC backbone for target expression
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||169111||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2400
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesEscherichia coli K12 MG1655
Insert Size (bp)581
MutationCodon optimized for E. coli
Entrez GenelacZ (a.k.a. b0344, ECK0341)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Plasmid design adapted from Daniel R, Rubens JR, Sarpeshkar R, Lu TK. Synthetic analog computation in living cells. Nature. 2013 May;497(7451):619–23.
Please visit https://doi.org/10.1101/2021.03.26.437171 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pNB200 was a gift from Edwin Wintermute (Addgene plasmid # 169111 ; http://n2t.net/addgene:169111 ; RRID:Addgene_169111)
For your References section:Low-cost anti-mycobacterial drug discovery using engineered E. coli. Bongaerts N, Edoo Z, Abukar AA, Song X, Sosa-Carrillo S, Haggenmueller S, Savigny J, Gontier S, Lindner AB, Wintermute EH. Nat Commun. 2022 Jul 7;13(1):3905. doi: 10.1038/s41467-022-31570-3. 10.1038/s41467-022-31570-3 PubMed 35798732