Nrf1-Myc pcDNA3.1(+)-C-Myc (NAWLVH106-AAAAAA)
(Plasmid
#169126)
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Purposeexpression of protein
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Depositing Lab
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Depositing OrganizationFrancis Crick Institute
Located in the United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 169126 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 169126-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 169126-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehNrf1
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Alt nameNF2L1_HUMAN
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Alt nameQ14494
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SpeciesH. sapiens (human)
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MutationNAWLVH106-AAAAAA
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Entrez GeneNFE2L1 (a.k.a. LCR-F1, NRF-1, NRF1, TCF11)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Unknown
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 169126-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 169126-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Nrf1-Myc pcDNA3.1(+)-C-Myc (NAWLVH106-AAAAAA) was a gift from Barbara Dirac-Svejstrup (Addgene plasmid # 169126 ; http://n2t.net/addgene:169126 ; RRID:Addgene_169126) -
For your References section:
DDI2 Is a Ubiquitin-Directed Endoprotease Responsible for Cleavage of Transcription Factor NRF1. Dirac-Svejstrup AB, Walker J, Faull P, Encheva V, Akimov V, Puglia M, Perkins D, Kumper S, Hunjan SS, Blagoev B, Snijders AP, Powell DJ, Svejstrup JQ. Mol Cell. 2020 Jul 16;79(2):332-341.e7. doi: 10.1016/j.molcel.2020.05.035. Epub 2020 Jun 9. 10.1016/j.molcel.2020.05.035 PubMed 32521225
