XP3 Xwnt-4
(Plasmid #16988)

• Depositing Lab: Randall Moon
• Publication: Xenopus Probe Plasmids (unpublished)

Backbone

• Vector backbone: pGem2
• Backbone size w/o insert (bp): 2900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Xwnt-4
• Alt name: Wnt-4
• Species: X. laevis (frog)
• Insert Size (bp): 1800
• GenBank ID: U13183
• Entrez Gene: wnt4 (a.k.a. Xwnt4, wnt-4, xwnt-4)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert: Xwnt-4 cDNA. Antisense probe cut with Nhe1 and transcribe with T7.
Sense probe cut with Sac1 and transcribe with SP6.

Plasmid History:
Constructed by Lynn McGrew on date of 11/90.