XE14 Xbhh-N
(Plasmid #16994)

• Depositing Lab: Randall Moon
• Publication: Xenopus Expression Plasmids (unpublished)

Backbone

• Vector backbone: pT7TS
• Backbone size w/o insert (bp): 3000
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Xbhh-N
• Alt name: Xbhh
• Alt name: hh1
• Alt name: banded hedgehog
• Species: X. laevis (frog)
• Entrez Gene: ihh (a.k.a. bhh, X-hh, bhh-a)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Expression Construct Xenopus Banded Hedgehog.
(a.k.a. Xbhh; hh1)

Insert: PCR of coding region, Genbank accession number U26404.
Antisense RNA: For in situ we use clone XIL in Bluescript KS. Linearize with Bam H1 and transcribe with T3.
Sense RNA: Linearize with Bam H1 and transcribe with T7.

References:
Ekker et al., Development 121, 2337-2347 (1995)
Lai et al., Development 121, 2349-2360 (1995)

Subclone Xbhh-N in pT7Ts.
Encodes amino terminal product with autoproteolytic cleavage. Linearize with BamH1 and transcribe with T7.