Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

(Plasmid #169995)


Item Catalog # Description Quantity Price (USD)
Plasmid 169995 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 3306
  • Total vector size (bp) 4803
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    kinesin family member 5B, isoform CRA_a
  • Alt name
    KIF5B K490clm
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    C7S, C65A, C168A, C174S, E215C, C294A, C330S, C421A
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHIS (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-17b_K490clm_E215C was a gift from Ron Vale (Addgene plasmid # 169995 ; ; RRID:Addgene_169995)
  • For your References section:

    Nanometer-accuracy distance measurements between fluorophores at the single-molecule level. Niekamp S, Sung J, Huynh W, Bhabha G, Vale RD, Stuurman N. Proc Natl Acad Sci U S A. 2019 Mar 5;116(10):4275-4284. doi: 10.1073/pnas.1815826116. Epub 2019 Feb 15. 10.1073/pnas.1815826116 PubMed 30770448