modRNAc0-Cas9-2A-GFP
(Plasmid
#170181)
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PurposeIVT template plasmid for making modified Cas9GFP RNA
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Depositing Lab
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Depositing OrganizationPennsylvania State University
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 170181 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 170181-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 170181-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonemodRNA cap0 plasmid
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Vector typeIVT template plasmid
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCas9-2A-GFP
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SpeciesSynthetic
- Promoter T7 promoter
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 promoter primer
- 3′ sequencing primer tatttaggtgacactatag
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.05.24.493220v1 for bioRxiv preprint.
DNA (Catalog # 170181-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 170181-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
modRNAc0-Cas9-2A-GFP was a gift from Xiaojun Lian (Addgene plasmid # 170181 ; http://n2t.net/addgene:170181 ; RRID:Addgene_170181) -
For your References section:
Robust genome editing via modRNA-based Cas9 or base editor in human pluripotent stem cells. Haideri T, Howells A, Jiang Y, Yang J, Bao X, Lian XL. Cell Rep Methods. 2022 Sep 7;2(9):100290. doi: 10.1016/j.crmeth.2022.100290. eCollection 2022 Sep 19. 10.1016/j.crmeth.2022.100290 PubMed 36160051
