Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


pBZ186-pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry
(Plasmid #170182)


Item Catalog # Description Quantity Price (USD)
Plasmid 170182 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    Addgene Plasmid #64323
  • Backbone manufacturer
    Kuehn lab
  • Modifications to backbone
    Vector was digested by NheI and BsrGI, mCherry was amplified from Addgene plasmid # 60954 (Weissman lab) and inserted into the digested vector backbone by Gibson assembly.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Promoter CBh
  • Tags / Fusion Proteins
    • 3XFlag (N terminal on backbone)
    • T2A (N terminal on backbone)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Genes encoding SpCas9 was obtained from previously reported plasmids (Addgene plasmid # 64323, Ralf Kühn lab); mCherry was amplified from Addgene plasmid # 60954 (Jonathan Weissman lab)

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBZ186-pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry was a gift from Hunter Fraser (Addgene plasmid # 170182 ; ; RRID:Addgene_170182)
  • For your References section:

    Bacterial Retrons Enable Precise Gene Editing in Human Cells. Zhao B, Chen SA, Lee J, Fraser HB. CRISPR J. 2022 Jan 24. doi: 10.1089/crispr.2021.0065. 10.1089/crispr.2021.0065 PubMed 35076284