pBluescript-Q22MS1-KO
(Plasmid #170312)

• Purpose: Plasmid for generating Tetrahymena thermophila somatic knockout strains for Q22MS1 with Paromomycin selection
• Depositing Lab: Andrew Carter
• Publication: Mali et al Science. 2021 Feb 26;371(6532):910-916. doi: 10.1126/science.abe0526.

Backbone

• Vector backbone: pBluescript
• Total vector size (bp): 7245
• Vector type: Tetrahymena somatic gene knockout vector
• Selectable markers: Paromomycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Q22MS1 knockout
• Alt name: -
• Species: Tetrahymena thermophila
• Insert Size (bp): 7245

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: actggccgtcgttttac
• 3′ sequencing primer: gtcctttgtcgatactg

Resource Information

• Supplemental Documents:
  • p2364_Q22MS1_Neo3 Paro_KO_Vector.gb
• A portion of this plasmid was derived from a plasmid made by: The original plasmids were received from Dr Kazufumi Mochizuki's laboratory.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Reference for source of plasmid backbone:
Kataoka, K., Schoeberl, U.E., and Mochizuki, K. (2010). Modules for C-terminal epitope tagging of Tetrahymena genes. J. Microbiol. Methods 82, 342–346.