tetO-HAND1
(Plasmid #170689)

• Purpose: doxycycline-inducible overexpression of HAND1
• Depositing Lab: Paul Burridge
• Publication: Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-2828(23)00106-2. doi: 10.1016/j.yjmcc.2023.06.005.

Backbone

• Vector backbone: FU-tetO-Gateway
• Backbone manufacturer: addgene plasmid #43914
• Backbone size w/o insert (bp): 8600
• Total vector size (bp): 9255
• Modifications to backbone: HAND1 ORF added to plasmid using Gateway cloning
• Vector type: Mammalian Expression, Lentiviral ; Doxycycline Inducible

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: HAND1
• Alt name: heart and neural crest derivatives expressed 1
• Alt name: Hxt
• Alt name: Thing1
• Species: H. sapiens (human)
• Insert Size (bp): 648
• Entrez Gene: HAND1 (a.k.a. Hxt, Thing1, bHLHa27, eHand)
• Promoter: TRE promoter, Tet-ON

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: LNCX; T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pOTB7-HAND1 obtained from Dharmacon (catalog ID: MHS6278-202827409)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was generated using Gateway cloning. attB sites were added to HEY2 via PCR reaction using ppOTB7-HAND1 as a template. Resulting fragements were recombined with pDONR221 to generate an entry clone. LR reaction was performed using the destination vector FU-tetO-Gateway (Addgene Plasmid #19778).