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pBS-ACTB-C200-GFPfr2
(Plasmid #170726)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170726 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 170726-D50 50 μg of DNA in Tris buffer $495
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DNA 170726-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pBlueScript II SK (+)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2898
  • Total vector size (bp) 3295
  • Vector type
    HR donor vector for human ACTB gene.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Donor sequence to ACTB gene for HR with the revised marker sequence
  • Species
    H. sapiens (human)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer taatacgactcactatagg
  • 3′ sequencing primer aattaaccctcactaaagg
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Using this plasmid as a substitute for pBS-ACTB-C200-GFPfr1, less non-specific amplification is observed in the downstream qPCR.
The following primer set can be used to detect the HR product.
GFPrev2-F: 5'-CTTCAAGATCCGCCACAACGT-3'
ACTB-common-R1: 5'-GTGCAATCAAAGTCCTCGGC-3'

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-ACTB-C200-GFPfr2 was a gift from Natsuko Chiba (Addgene plasmid # 170726 ; http://n2t.net/addgene:170726 ; RRID:Addgene_170726)
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