pET-15b(+) Toxoplasma gondii (delta prenylation motif)
(Plasmid
#170789)
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PurposeExpresses pET-15b(+) Toxoplasma gondii (delta prenylation motif)
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 170789 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET-15B
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Backbone manufacturerNovagen Inc., Madison, WI
- Backbone size w/o insert (bp) 5708
- Total vector size (bp) 6888
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Modifications to backboneNA
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameToxoplasma gondii PRL
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Alt nameProtein tyrosine phosphatase
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SpeciesToxoplasma gondii
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Insert Size (bp)1197
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MutationDeleted nucleotides coding for 4 C-terminal amino acids
- Promoter AmpR, lacI
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Tag
/ Fusion Protein
- N-terminal His-tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer pET-15b Fwd
- 3′ sequencing primer pGEX Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-15b(+) Toxoplasma gondii (delta prenylation motif) was a gift from Kalle Gehring (Addgene plasmid # 170789 ; http://n2t.net/addgene:170789 ; RRID:Addgene_170789)
Map uploaded by the depositor.
