pYI012
(Plasmid
#170841)
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PurposepGH3.17::GUSPlus::tOCS
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 170841 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepYI001
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Streptomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGH3.17p
- Promoter GH3.17p
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ATCCAAGCTCAAGCTAAGCTCATAAACATTTACCTTTCATGG
- 3′ sequencing primer TAGAAATTTACCCTCAGATCTACCATtTtctgaaagcagacacaaacaaagc (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2021.06.16.448628 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYI012 was a gift from Christian Fankhauser (Addgene plasmid # 170841 ; http://n2t.net/addgene:170841 ; RRID:Addgene_170841) -
For your References section:
A combination of plasma membrane sterol biosynthesis and autophagy is required for shade-induced hypocotyl elongation. Ince YC, Krahmer J, Fiorucci AS, Trevisan M, Galvao VC, Wigger L, Pradervand S, Fouillen L, Van Delft P, Genva M, Mongrand S, Gallart-Ayala H, Ivanisevic J, Fankhauser C. Nat Commun. 2022 Oct 10;13(1):5659. doi: 10.1038/s41467-022-33384-9. 10.1038/s41467-022-33384-9 PubMed 36216814
