ZE44 myc-Dapper-2 pCS2P+
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17085||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Vector typeZebrafish Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
SpeciesD. rerio (zebrafish)
Entrez Genedact2 (a.k.a. Amin, dact, dapper2, dpr2, frd2, frodo2, ik:tdsubc_1d9, wu:fe24f12, xx:tdsubc_1d9, zgc:109729, zgc:152832)
/ Fusion Protein
- myc (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Dishevelled associated protein. tagged with c-myc epitope at amino terminus. cloned into Cla/EcoR1 sites.
Notes for Use: For functional synthetic RNA cut NotI, transcribe SP6.
Select clones with amp.
Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ZE44 myc-Dapper-2 pCS2P+ was a gift from Randall Moon (Addgene plasmid # 17085 ; http://n2t.net/addgene:17085 ; RRID:Addgene_17085)