|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17090||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Vector typeZebrafish Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
SpeciesD. rerio (zebrafish)
Insert Size (bp)2000
Entrez Genesp5l (a.k.a. Spr2, YF-335, cb577, fc23b01, s, sb:cb57, sb:cb577, wu:fc23b01)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Gilbert Weidinger, Ph.D
Zebrafish sp5-like (sp5l) cDNA (likely full-length) in pSPORT1.
Clone 27i2 from the microarray screen 1st round (Matthew clark normalized late somitogenesis / 24 hpf / liver library). Expressed strongly in broad marginal region during gastrulation. Everywhere except head at bud, then rapidly declines to stay in tailbud and lateral mesoderm (or edge of neural plate).
For antisense: Asp718, SP6
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ZE49 pSPORT1-Sp5-l was a gift from Randall Moon (Addgene plasmid # 17090 ; http://n2t.net/addgene:17090 ; RRID:Addgene_17090)