pUC19_HR-BirA-FLAG_Ago2
(Plasmid
#170918)
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PurposeEncodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago2
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 170918 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUC19
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Backbone manufacturerJoachim Messing Lab (Addgene #50005)
- Backbone size w/o insert (bp) 2660
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBirA-FLAG
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SpeciesM. musculus (mouse), Synthetic
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Insert Size (bp)2135
- Promoter lac
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer M13_Fw: TGTAAAACGACGGCCAG
- 3′ sequencing primer M13_Rv: CAGGAAACAGCTATGAC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC19_HR-BirA-FLAG_Ago2 was a gift from Constance Ciaudo (Addgene plasmid # 170918 ; http://n2t.net/addgene:170918 ; RRID:Addgene_170918) -
For your References section:
AGO1 regulates pericentromeric regions in mouse embryonic stem cells. Muller M, Fah T, Schaefer M, Hermes V, Luitz J, Stalder P, Arora R, Ngondo RP, Ciaudo C. Life Sci Alliance. 2022 Mar 2;5(6). pii: 5/6/e202101277. doi: 10.26508/lsa.202101277. Print 2022 Jun. 10.26508/lsa.202101277 PubMed 35236760
