pX458_sgAgo1_Nterm
(Plasmid #170921)

• Purpose: Expresses spCas9 and sgRNA targeting the N-terminus of Ago1 for N-terminal tagging
• Depositing Lab: Constance Ciaudo
• Publication: Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii: 5/6/e202101277. doi: 10.26508/lsa.202101277. Print 2022 Jun.

Backbone

• Vector backbone: pX458
• Backbone manufacturer: Feng Zhang Lab (Addgene# 48138)
• Backbone size w/o insert (bp): 9272
• Vector type: Mammalian Expression, CRISPR
• Selectable markers: EGFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: sgAgo1
• gRNA/shRNA sequence: TGGGATGGAAGCGGGACCCT
• Species: M. musculus (mouse)
• Insert Size (bp): 20
• Entrez Gene: Ago1 (a.k.a. Eif2c, Eif2c1)
• Promoter: hU6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BbsI (destroyed during cloning)
• 3′ cloning site: BbsI (destroyed during cloning)
• 5′ sequencing primer: hU6: GAGGGCCTATTTCCCATGATT

Resource Information

• Supplemental Documents:
  • pX458_sgAgo1_Nterm.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry