M15 pCS-twhh-nBeta gal
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17152||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepCS-NBeta gal
Growth in Bacteria
Gene/Insert nametwhh promoter
Alt nametiggy-winkle hedgehog
SpeciesD. rerio (zebrafish)
Insert Size (bp)5200
Entrez Geneshhb (a.k.a. twh, twhh)
/ Fusion Protein
- nuclear beta-gal (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (unknown if destroyed)
- 3′ cloning site HindIII (destroyed during cloning)
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Insert: twhh promoter (5.2 Kb), nuclear Beta gal reporter (3 Kb)
Notes for Use:
Gives notochord-specific expression of Beta-gal.
Citation: Du et al., 1997, J. Cell Biology.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:M15 pCS-twhh-nBeta gal was a gift from Randall Moon (Addgene plasmid # 17152 ; http://n2t.net/addgene:17152 ; RRID:Addgene_17152)
Map uploaded by the depositor.